RAYBIO HUMAN CYTOKINE ANTIBODY ARRAY C SERIES 2000

Additional file 3 Technical characteristics of the immunoassays used for validation. Click here for file 29K, docx. The present study was performed to apply a serum antibody microarray to screen for differentially regulated cytokines in Parkinson’s disease PD , multiple system atrophy MSA , progressive supranuclear palsy PSP and corticobasal syndrome CBS. Acknowledgements The authors wish to thank all the patients and controls who contributed samples for this study, Kathrin Schanda for excellent technical assistance and Dr. All authors read and approved the final manuscript. Diabetes type 2, metabolic disease, reproduction 1.

The present study was performed to apply a serum antibody microarray to screen for differentially regulated cytokines in Parkinson’s disease PD , multiple system atrophy MSA , progressive supranuclear palsy PSP and corticobasal syndrome CBS. Serum levels of interleukin-6 are elevated in patients with Parkinson’s disease and correlate with physical performance. Conclusions In our unbiased cytokine array based screening approach and validation by a different immunoassay only two of cytokines were significantly altered between patients and controls. To our knowledge this is the first study using an unbiased cytokine microarray analysis approach to identify potential serum biomarkers for the discrimination of neurodegenerative parkinsonian disorders. Author information Article notes Copyright and License information Disclaimer. Abstract Background Microarray technology may offer a new opportunity to gain insight into disease-specific global protein expression profiles. Also in the subgroups of early untreated patients PDGF was elevated to the same extent as in the whole groups.

Cell migration, leukocyte migration, cell motility. Prolactin response as an index of dopaminergic receptor function in Parkinson’s disease. Beside this methodological concern, our results suggest that the serum prolactin levels are influenced by dopaminergic antiparkinsonian treatment, but not the patient group; dopaminergic antiparkinsonian treatment remained the only significant variable on prolactin levels in a multivariate analysis and patients with no such treatment had similar serum prolactin levels to controls.

Interestingly, the most recent of these studies also included 11 demented PD patients and, in line with our results, they found an increase in PDGF-BB levels in their plasma [ 10 ]. Click here for file 29K, docx. Cell chemotaxis, response to steroid hormone stimulus.

Experimental studies from the s demonstrated that PDGF-BB acts as a trophic factor for rat and human mesencephalic dopaminergic neurons promoting gene expression, survival and neurite outgrowth in culture [ 2021 ]. Identification of serum biomarkers for the discrimination of movement disorders by antibody arrays in the screening cohort.

Acknowledgements The authors wish to thank all the patients and controls who contributed samples for this study, Kathrin Schanda for excellent technical assistance and Dr. Results of cytokine microarray experiment. In order to exclude an effect of age-related non-neurological diseases on serum cytokine levels we have not only included 63 age and sex matched healthy blood donors HCbut also 36 patients with internal diseases INC in our control group.

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Platelet-derived growth factor promotes survival of rat and human mesencephalic dopaminergic neurons in culture. Comprehensive analysis of chemotactic factors for bone marrow mesenchymal stem cells. Nadia Stefanova for her very helpful comments. Biological fluid biomarkers in neurodegenerative parkinsonism. Signals were normalized using internal, positive and negative controls included on the array. In our cohort of patients with neurodegenerative parkinsonian syndromes, there was no association of PDGF-BB levels with the disease duration or the Hoehn and Yahr score.

A serum protein-based algorithm for the detection of Alzheimer disease. National Center for Biotechnology InformationU. The array glass slides were washed, incubated with a biotin-conjugated anti-cytokine mix for 2 hours, washed again, and developed for 2 hours with Cy3-conjugated streptavidin. Biomarkers for Parkinson’s disease.

AMd and all patients gave written informed consent to the study protocol. Open in a separate window. Individual data points are shown as circles and horizontal bars indicate medians. Discussion To our knowledge this is the first study using an unbiased cytokine microarray analysis approach to identify potential serum biomarkers for the discrimination of neurodegenerative parkinsonian disorders.

Individual data points are shown as circles or triangles and horizontal bars indicate medians.

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Mechanism of action and in vivo role of platelet-derived growth factor. Cell chemotaxis, response to steroid hormone stimulus 2. Diabetes type 2, metabolic disease, reproduction 1. This could explain why PDGF-BB was also detectable in our control sera to a marked extent, which accounts for the suboptimal differentiation of neurodegenerative Parkinsonian syndromes from controls. The diagnosis of dementia due to Alzheimer’s disease: Since PDGF-BB has several important functions in the peripheral circulation such as mitogenic and chemotactic effects on mesenchymal stem cells [ 26 – 29 ], it is more likely that the increased serum PDGF-BB levels observed in our study might reflect a response to pathological changes in the periphery.

Additional file 3 Technical characteristics of the immunoassays used for validation.

Human Cytokine Array C2000 (4) – AAH-CYT-2000-4 – 1 kit – RayBiotech – assays & kits

Plasma epidermal growth factor levels predict cognitive decline in Parkinson disease. A post-hoc analysis revealed no statistically significant differences for these cytokines between INC and HC within the control group.

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Immune response, immunity and defense 7. Chemotaxis, taxis, locomotory behavior. Furthermore, biomarkers could be a helpful tool to evaluate the progression eaybio the severity of PD. Immune response, immunity and defense.

Table 3 Validation of the serum cytokine array experiment in the initial screening cohort. Serum levels of brain-derived neurotrophic factor correlate with motor impairment in Parkinson’s disease. Platelet-derived growth factor BB mediates the tropism of human mesenchymal stem cells for malignant gliomas.

In a second step cytojine from the microarray screening experiment were evaluated by different immunoassays.

Human Cytokine Array C2000

However, peripheral blood is much easier to obtain and not all patients are willing to undergo lumbar puncture. Positive regulation of signal transduction. These seven cytokines were selected based on the results from the microarray experiments, the availability of commercial available test kits and the amount of serum left.

Therefore a longitudinal anibody is now needed to address this important question.

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DJ-1 and alpha-synuclein in human cerebrospinal fluid as biomarkers of Parkinson’s disease. Published online Nov Platelet-derived growth factor exerts trophic effects on rat striatal DARPPcontaining neurons in culture.

The present study was performed to apply a serum antibody microarray to screen for differentially regulated cytokines in Parkinson’s disease PDmultiple system atrophy MSAprogressive supranuclear palsy PSP and corticobasal syndrome CBS. Microarray technology may offer a new opportunity to gain insight into disease-specific global protein expression profiles.

The authors wish to thank all the patients and controls who contributed samples for this study, Kathrin Schanda for excellent technical assistance and Dr. To our knowledge this is the first study using an unbiased cytokine microarray analysis approach to identify potential serum biomarkers for the discrimination of neurodegenerative parkinsonian disorders. Signals were scanned with a GenePix B scanner. Positive regulation of signal transduction 1.

Five out of 16 plasma signaling proteins are enhanced in plasma of patients with mild cognitive impairment and Alzheimer’s disease.

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